How to make bakposev
How to make bakposev
Bakposev (bacteriological culture) islaboratory microbiological study of human biomaterial. It is carried out by sowing on nutrient media at the established temperature regime. The aim of the study is to determine pathogenic and conditionally pathogenic bacteria for the selection of treatment tactics.
Instructions
1
Bakposev is widely used in urology,gynecology, surgery, oncology, otolaryngology and other fields of medicine. Indications for the analysis are inflammatory processes, suspected sepsis. For biological culture, the biological environment of the body is taken: phlegm, nasopharyngeal mucus, sputum (secret of the bronchial tree), feces, mucus of the cervical canal, urethra, secretion of the prostate, blood, urine, cerebrospinal fluid, bile, breast milk, inflammatory foci, cysts , wound detachable.
2
The quality and effectiveness of the potato will be, independ on the correctness of the sampling of the biomaterial. It is necessary to use sterile dishes and sterile instruments that are given to patients in a bacteriological laboratory. Blood and urine should be collected in dry test tubes, the rest of the biomaterials - in dishes with nutrient medium. After the collection of material, it is necessary to ensure rapid delivery to the laboratory.
3
Urine in the amount of 10-15 ml is collected in a sterileutensils, an average portion of morning urine is needed for analysis. Biomaterial should be delivered to the baklaboratoriya within two hours. A smear from the throat and nose is taken by the laboratory assistant, before taking the test, do not rinse your mouth, brush your teeth, bury in the nose with disinfectant solutions. You can not drink and eat.
4
Fence removal in an amount of 15-30 gin the morning. Do not allow urine to enter the feces. The biomaterial should be delivered to the baklaboratory for 5 hours. Sputum is collected in a sterile container in the morning on an empty stomach during an attack of cough accompanied by the separation of mucus. Before taking the analysis, you need to brush your teeth and rinse your mouth with boiled water. The material should be delivered to the baklaboratoriya within an hour.
5
Blood for bacterosseum is taken by a laboratory technician beforeantibiotic therapy in a sterile tube in an amount of 15 ml (in adults) and 5 ml (in children). The collection of genital secretions in women is carried out no earlier than 2 weeks after menstruation. It is advisable not to urinate for 2 hours. Before taking a sample, men are not recommended to empty the bladder for 5-6 hours.
6
In the bacteriological laboratory, the material forStudies are placed on nutrient media, depending on the search for a particular pathogen. Then they are sent to a thermostat, which creates favorable conditions for the growth and reproduction of bacteria. After a certain time with the help of a microscope, a control inspection of the bacterial colonies, which are called "culture of microorganisms", is carried out. Assess the shape, color, density, the ability to decompose organic and inorganic compounds. Count the number and concentration of pathogens.
