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Laboratory study of feces for dysbiosis

Analysis of stool for dysbacteriosis is carried out as followsway. Cal is repeatedly diluted with water, obtaining a ratio of 1: 100,000. Then the biomaterial in the amount of 0.1 ml is sown on special nutrient media, each of which is intended for growing a certain type of bacteria. After a few days, colonies of microorganisms grow, which are then studied under a microscope. Identify the types of bacteria, count the number of their colonies on each medium. The obtained data are reflected in the analysis, which is a table in which the number of bacteria of a certain type is indicated (the norm and the actual content). The number of bacteria: pathogenic flora is absent, the fungi absent, proteus absent, the escherichia coli 1-7x10 8 , Escherichia coli, which has weakly expressed enzymatic properties - less than 10%, hemolysing escherichia coli - absent, lactose-negative enterobacteria - 5%, coccal forms - less than 25%, enterococci - 1x10 ^ 5-10 ^ 8, bifidobacteria - 10 ^ 9 and more, lactobacilli - 1x 10 ^ 7-10 ^ 8.

How to take a stool test

A reliable result can be obtained only inif the biomaterial is properly collected. In the feces should not get foreign impurities, it is necessary that he contacted the air as little as possible. It is not recommended to take the test during the period of taking medications containing any microflora. To collect stool, you must use a sterile container, which is issued in the laboratory. Feces are collected only after the natural process of defecation. Do not take laxatives, do enemas to cause the appearance of stools. Do not allow urine to enter the feces. Feces for analysis are collected with a special spoon or spatula, which is attached to a sterile test tube. They must be taken in small quantities from different places of stool. Immediately after collecting the biomaterial, the container is closed with a lid and delivered to the laboratory within 2 hours. It must be taken into account that even exact observance of the procedure can not guarantee an accurate estimate of the amount and ratio of microflora. This method of analysis allows you to count bacteria that are in the lumen of the intestine. However, microorganisms are located on the intestinal mucosa, with the help of this method, their abundance and correlation can not be determined. Since dysbiosis can accompany more serious diseases, other diagnostic procedures are also performed when this diagnosis is confirmed.